WHO Hand Sanitizer Formula is Free and Public Available


WHO Hand Sanitizer Formula

WHO Hand Sanitizer Formula

Introduction: This Guide to Local Production of WHO-recommended Handrub Formulations is separated into two discrete but interrelated sections:

Part A provides a practical guide for use at the pharmacy bench during the formulation’s actual preparation. Users may want to display the material on the wall of the production unit.

Part B summarizes some essential background technical information and is taken from WHO Guidelines on Hand Hygiene in Health Care (2009).

The user has access to critical safety and cost information and supplementary material relating to Part B dispensers and distribution.



Part A is intended to guide a local producer in the actual preparation
of the formulation. Essentially, How to make your own hand sanitizer

Materials required (small volume production)


•   Ethanol 96%

•   Hydrogen peroxide 3%

•   Glycerol 98%

•   Sterile distilled or boiled cold water

•   Isopropyl alcohol 99.8%

•   Hydrogen peroxide 3%

•   Glycerol 98%

•   Sterile filtered or boiled cold water


  • 10-liter glass or plastic bottles with screw-threaded stoppers, or
  • 50-liter plastic tanks (preferably in polypropylene or high-density polyethylene, translucent to see the liquid level) or
  • Stainless steel tanks with a capacity of 80–100 liters (for mixing without overflowing)
  • Wooden, plastic, or metal paddles for mixing
  • Measuring cylinders and measuring jugs
  • Plastic or metal funnel
  • 100 ml plastic bottles with leak-proof tops
  • 500 ml glass or plastic bottles with screw tops

An alcoholometer: the temperature scale is at the bottom and the ethanol concentration (percentage v/v) at the top


  • Glycerol: used as a humectant, but other emollients may be used for skincare, provided that they are cheap, widely available, and miscible in water and alcohol and do not add to toxicity or promote allergy.
  • Hydrogen peroxide: used to inactivate contaminating bacterial spores in the solution and is not an active substance for hand antisepsis.
  • Any further additive to both formulations should be clearly labeled and be non-toxic in case of accidental.
  • A colorant may be added to allow differentiation from other fluids. Still, it should not add to toxicity, promote allergy, or interfere with antimicrobial The addition of perfumes or dyes is not recommended due to the risk of allergic reactions.

Full Document with all details can be downloaded HERE